Basic Biology of Aging at the University of Washington

Invertebrate Longevity & Healthspan Core

Description

This Core provides methods, tools, and expertise for analyzing longevity and healthspan in the common invertebrate model organisms used for aging-related studies.  This includes extensive measures for determining longevity and healthspan in budding yeast, and Caenorhabditis elegans, and support for experimental design in these organisms as well as Drosophila melanogaster

Our overarching goal is to provide training, resources, and access to experimental methods in aging research that would otherwise be unavailable or difficult to obtain for investigators in the Gerontological community at large.  Core resources are available to researchers at institutions throughout the United States and internationally and will generally be provided as a collaborative resource, rather than purely a service Core.  As such, Core personnel will work closely with Core users to develop the optimal approaches and experimental design for their specific project and the scientific questions they wish to address.  When appropriate for the project, Core personnel will also work with Cores at other Nathan Shock Centers (NSCs) to ensure that Core users receive the best support for their project.   

Costs for Core C services are shared between our NSC and user fees, with the specific details to be negotiated on a case-by-case basis.  The goal of this Core is to maintain user fees at a minimal cost.  When mutually agreeable and feasible within the funding constraints, we will perform services at no cost, aside from the cost of necessary reagents or supplies not already owned by the Core

Services available:

  • Consultation on experimental design for invertebrate lifespan analysis
  • Statistical support and power analysis

Yeast aging:

  • Replicative lifespan analysis
  • Chronological lifespan analysis
  • Quantitative growth rate measurements
  • Strain construction
  • FACS analysis
  • Microfluidic based studies
  • Fluorescence and light microscopy

C. elegans aging:

  • Lifespan analysis
  • Fecundity
  • Developmental rate
  • Stress resistance (hypoxia, hyperoxia, oxidative stress, heat, etc.)
  • Motility
  • Strain construction
  • Fluorescence and light microscopy

Shared Equipment and Resources


In collaboration with the UW Healthy Aging and Longevity (HALo) Research Institute, the Core provides access to a variety of equipment and shared resources.  These include:

  • C. elegans injection microscope and needle puller
  • COPAS Biosort Wormsorter
  • Bioscreen C MBR
  • Zeiss Lumar Dissecting Microscope
  • Dissecting and fluorescence microscopes suitable for yeast and worm work
  • A variety of yeast strains and worm RNAi clones (contact with specific requests)

Personnel

Matt Kaeberlein, Director, is an expert in the basic biology of aging.  His lab has published extensively using both yeast and C. elegans models of aging.  All inquiries should be made to Dr. Kaeberlein.

Albert Folch, co-investigator, is an expert in microfluidic applications and bioengineering.  He provides consultation on development of new microfluidic technologies for aging-related research.

Daniel Promislow, co-investigator, is an expert in evolutionary biology of aging and provides expertise in fruit fly biology and statistical analysis.