Basic Biology of Aging at the University of Washington

Proteomics Core

Description

The Proteomics Core of the UW Nathan Shock Center is designed to provide the Aging Research Community with access to state-of-the-art proteomics technologies. The Core has been at the forefront of developing technologies to perform large-scale targeted proteomics assays that generate near complete data matrices, accurate quantitation, and high reproducibility – even across multiple laboratories. The primary goal of the Proteomics Core is to apply these improved methods to studies of aging, to obtain accurate quantitative measurements of peptides from samples provided by its users, and to aid geroscience researchers in linking these high-dimensional data to biologically meaningful outcomes. In addition, the Core will apply new assays well- suited to aging as they are developed. This Core also works closely with Core B (Metabolite Phenotypes of Aging), which will share some of the same facilities.

Services available:

  • Support with design and execution of experiments using proteomics to ask aging–related questions
  • Guidance and assistance in sample preparation for proteomics
  • Targeted and discovery proteomics data collection
  • Measurements of peptide abundance and half-life by mass spectrometry
  • Software tools and support for the analysis of proteomics data. These include Skyline for
  • targeted and SRM/PRM proteomics and Topograph for stable isotope half-life measurements (see https://skyline.gs.washington.edu/labkey/project/home/begin.view?).


Several workshops on software tools are held each year.


Personnel
Mike MacCoss and Daniel Promislow