OrganismSaccharomyces cerevisiae (replicative)
Aging PhenotypeLife-span extension
Allele TypeOverexpression
StrainW303, PSY142
DescriptionOverexpression of UTH4 from the ADH promoter extends life-span in PSY142 by about 25% (Kennedy et al., 1997) and extends life-span in W303R by about 20% (Kaeberlein and Guarente, 2002). Deletion of UTH4 shortens life span by about 50% in W303.
Gene FunctionPost-transcriptional repressor of HO (Tomofumi et al, 2001).
Other PhenotypesUTH4 mutants are temperature sensitive (Kikuchi et al., 1994) and hypersensitive to mating pheromone (Chen and Kurjan, 1997). Null mutants also show increased silencing at telomeres and decreased rDNA silencing (Sinclair et al., 1998). Overexpression of UTH4 suppresses the temperature sensitive phenotype of a POP2 mutant (Hata et al., 1997). Deletion of MPT5 is synthetically lethal with mutation of either SWI4, SWI6 or CCR4 in an ssd1-d background (Kaeberlein and Guarente, 2002). Uth4p is required for relocalization of the SIR complex to the nucleolus in sir4-42 strain (Kennedy et al., 1997).
HomologsS.c. PUF4, YLL013, PUF2, JSN1
S.p. Spac6g9.14, Spac222.02c, Spac4g8.03c, Spcc1682.08c, Spbp35g2.14, Spac4g9.05, Spbc56f2.08c
C.e. W06B11.2, puf-8, Y45F10A.2, fbf-1, fbf-2, F54C9.9, puf-7, Y48G1BL.E, puf-6, M4.2
D.m. PUM
H.s. KIAA0235, KIAA0099
Primary ReferenceKennedy, B. K., Gotta, M., Sinclair, D. A., Mills, K., McNabb, D. S., Murthy, M., Pak, S. M., Laroche, T., Gasser, S. M., and Guarente, L. (1997). Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life sp [Abstract]
Kaeberlein, M., and Guarente, L. (2002). Saccharomyces cerevisiae MPT5 and SSD1 Function in Parallel Pathways to Promote Cell Wall Integrity. Genetics 160, 83-95. [Abstract]
Other ReferencesChen, T., and Kurjan, J. (1997). Saccharomyces cerevisiae Mpt5p interacts with Sst2p and plays roles in pheromone sensitivity and recovery from pheromone arrest. Mol Cell Biol 17, 3429-39. [Abstract]
Hata, H., Mitsui, H., Liu, H., Bai, Y., Denis, C. L., Shimizu, Y., and Sakai, A. (1998). Dhh1p, a putative RNA helicase, associates with the general transcription factors Pop2p and Ccr4p from Saccharomyces cerevisiae. Genetics 148, 571-9. [Abstract]
Kennedy, B. K., Austriaco, N. R., Zhang, J., and Guarente, L. (1995). Mutation in the silencing gene SIR4 can delay aging in S. cerevisiae. Cell 80, 485-96. [Abstract]
Kikuchi, Y., Oka, Y., Kobayashi, M., Uesono, Y., Toh-e, A., and Kikuchi, A. (1994). A new yeast gene, HTR1, required for growth at high temperature, is needed for recovery from mating pheromone-induced G1 arrest. Mol Gen Genet 245, 107-16. [Abstract]
Sinclair, D. A., Mills, K., and Guarente, L. (1998). Molecular mechanisms of yeast aging. Trends Biochem Sci 23, 131-4. [Abstract]
Tadauchi, T., Matsumoto, K., Herskowitz, I., and Irie, K. (2001). Post-transcriptional regulation through the HO 3'-UTR by Mpt5, a yeast homolog of Pumilio and FBF. Embo J 20, 552-61. [Abstract]
Relevant LinksYPD:
KeywordsSilencing, nucleolus, rDNA, telomeres, yeast, replicative senescence, Saccharomyces, cerevisiae, RNA, starvation resistance, cell wall, stress