OrganismSaccharomyces cerevisiae (replicative)
Aging PhenotypeLife-span extension
Allele TypeDeletion
StrainW303R, K2307, W3031-A
DescriptionDeletion of FOB1 extends life span by approximately 30% in W303 and 50% in K2307 (Defossez et al., 1999). Mutation of FOB1 also supresses the short life span of a SIR2 mutant (Kaeberlein et al., 1999). Loss of Fob1p function dramatically decreases the formation of extrachromosomal rDNA circles, thereby lengthening lifespan (Defossez et al., 1999).
Gene FunctionRequired for formation of the replication fork block in rDNA (Kobayashi and Horiuchi, 1996).
Other PhenotypesFOB1 mutants have decreased rDNA recombination and are unable to increase or decrease the number of rDNA repeats (Kobayashi et al., 1998).
Primary ReferenceDefossez, P. A., Prusty, R., Kaeberlein, M., Lin, S. J., Ferrigno, P., Silver, P. A., Keil, R. L., and Guarente, L. (1999). Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell 3, 447-55. [Abstract]
Other ReferencesKaeberlein, M., McVey, M., and Guarente, L. (1999). The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev 13, 2570-80. [Abstract]
Kobayashi, T., and Horiuchi, T. (1996). A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities. Genes Cells 1, 465-74. [Abstract]
Kobayashi, T., Heck, D. J., Nomura, M., and Horiuchi, T. (1998). Expansion and contraction of ribosomal DNA repeats in Saccharomyces cerevisiae: requirement of replication fork blocking (Fob1) protein and the role of RNA polymerase I. Genes Dev 12, 3821-3 [Abstract]
Relevant LinksYPD:
KeywordsrDNA, recombination, DNA replication, replicative senescence, Saccharomyces, cerevisiae, nucleolus, yeast