InterventionEctopic extrachromosomal rDNA circle (ERC) induction.
Alternate Name
OrganismSaccharomyces cerevisiae (replicative)
Aging PhenotypeShortened life-span
Allele TypeN/A
DescriptionERCs, circular multimers of the rDNA repeat, naturally accumulate to very high levels in old mother cells. Ectopic production of an ERC in virgin cells reduces life span by approximately 40% (Sinclair and Guarente, 1997).
Gene FunctionN/A
Other PhenotypesERCs segregate preferentially to the mother cell, although this asymmetric inheritance pattern breaks down as cells age (Sinclair and Guarente, 1997).
ERC release prevents life-span extension by deletion of FOB1 (Defossez et al., 1999) and shortens life-span to the level of a SIR4 mutant (Kaeberlein et al., 2000).
Primary ReferenceSinclair, D. A., and Guarente, L. (1997). Extrachromosomal rDNA circles--a cause of aging in yeast. Cell 91, 1033-42. [Abstract]
Other ReferencesDefossez, P. A., Prusty, R., Kaeberlein, M., Lin, S. J., Ferrigno, P., Silver, P. A., Keil, R. L., and Guarente, L. (1999). Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell 3, 447-55. [Abstract]
Kaeberlein, M., McVey, M., and Guarente, L. (1999). The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev 13, 2570-80. [Abstract]
Relevant LinksNone
KeywordsrDNA, replicative senescence, Saccharomyces,cerevisiae, nucleolus, DNA, yeast