NameRPD3
OrganismDrosophila melanogaster
Aging PhenotypeLife-span extension
Allele TypeRecessive
StrainCanton-S
DescriptionFlies heterozygous for a hypomorphic allele of rpd3 have life-spans extended by 33% (males) and 52% (females) (Rogina et al., 2002).
Gene FunctionHistone deacetylase
Other PhenotypesExpression of Sir2 is increased 2-fold in rpd3 flies (Rogina et al., 2002). Caloric restriction in flies results in decreased transcription of RPD3 (Pletcher et al., 2002) and fails to further extend the life-span of rpd3 flies (Rogina et al., 2002).
Deletion of RPD3 extends life-span in yeast (Kim et al., 1999) and increases SIR2-dependent rDNA silencing (Smith et al., 1999). Overexpression of SIR2 extends life-span in yeast (Kaeberlein et al., 1999) and life-span extension by deletion of RPD3 is likely to be the result of increased SIR2 activity.
HomologsS.c. RPD3, HOS2, HOS1, HDA1
S.p. clr6, hda1, clr3
C.e. hda-1, RO6C1.1, hda-2, C10E2.3, F41H10.6
D.m. CG2128, HDAC3, HDAC2, CG1770, CG10899
M.m. Hdac2, Hdac3, Mm.29854, HDAC7, Hdac5, Mm.22665
H.s. HDAC2, HDAC1, HDAC3, HSA277724, DKFZP586J0917, HDAC7,
Primary ReferenceRogina, B., Helfand, S. L., and Frankel, S. (2002). Longevity regulation by Drosophila Rpd3 deacetylase and caloric restriction. Science 298, 1745. [Abstract]
Other ReferencesPletcher, S. D., Macdonald, S. J., Marguerie, R., Certa, U., Stearns, S. C., Goldstein, D. B., and Partridge, L. (2002). Genome-wide transcript profiles in aging and calorically restricted Drosophila melanogaster. Curr Biol 12, 712-23. [Abstract]
Smith, J. S., Caputo, E., and Boeke, J. D. (1999). A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19, 3184-97. [Abstract]
Kim, S., Benguria, A., Lai, C. Y., and Jazwinski, S. M. (1999). Modulation of life-span by histone deacetylase genes in Saccharomyces cerevisiae. Mol Biol Cell 10, 3125-36. [Abstract]
Kaeberlein, M., McVey, M., and Guarente, L. (1999). The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev 13, 2570-80. [Abstract]
Relevant LinksLocusLink: http://www.ncbi.nih.gov/LocusLink/LocRpt.cgi?l=38565
FlyBase: http://flybase.bio.indiana.edu/.bin/fbidq.html?FBgn0015805
Keywordstranscription, silencing, calorie restriction, chromatin