Nameclk-1
OrganismCaenorhabditis elegans
Aging PhenotypeLife-span extension
Allele TypeRecessive
StrainBristol N2
DescriptionLoss of function mutation increases adult life-span by 30%. Mutation of both clk-1 and daf-2 results in a nearly 5-fold (500%) increase in life-span (Lakowski and Hekimi, 1996).
Overexpression of clk-1 increases mitochondrial activity and shortens life-span (Felkai et al., 1999).
Gene FunctionRequired for ubiquinone biosynthesis. Has strong similarity to the human ubiquinone biosynthesis protein COQ7 / clk-1.
Other PhenotypesMutations in clk-1 are highly pleiotropic resulting in an average lengthening of embryonic development, post-embryonic development, and adult rhythmic behaviors such as defecation, swimming, and pharyngeal pumping (Wong et al., 1995).
Clk-1 protein binds the mitochonrdial O(L) region and may regulate replication of mitochondrial DNA (Gorbunova and Seluanov, 2002).
clk-1 mutants require a dietary source of coenzyme Q (Jonassen et al., 2001).
HomologsS.c. CAT5
S.p. Spbc337.15c
D.m. COQ7
R.n. COQ7
M.m. COQ7
H.s. COQ7
Primary ReferenceLakowski, B., and Hekimi, S. (1996). Determination of life-span in Caenorhabditis elegans by four clock genes. Science 272, 1010-3. [Abstract]
Other ReferencesFelkai, S., Ewbank, J. J., Lemieux, J., Labbe, J. C., Brown, G. G., and Hekimi, S. (1999). CLK-1 controls respiration, behavior and aging in the nematode Caenorhabditis elegans. EMBO Journal 18, 1783-1792. [Abstract]
Jonassen, T., Proft, M., Randez-Gil, F., Schultz, J. R., Marbois, B. N., Entian, K. D., and Clarke, C. F. (1998). Yeast Clk-1 homologue (Coq7/Cat5) is a mitochondrial protein in coenzyme Q synthesis. J Biol Chem 273, 3351-7. [Abstract]
Jonassen, T., Larsen, P. L., and Clarke, C. F. (2001). A dietary source of coenzyme Q is essential for growth of long-lived Caenorhabditis elegans clk-1 mutants. Proc Natl Acad Sci U S A 98, 421-6. [Abstract]
Wong, A., Boutis, P., and Hekimi, S. (1995). Mutations in the clk-1 gene of Caenorhabditis elegans affect developmental and behavioral timing. Genetics 139, 1247-59. [Abstract]
Gorbunova, V., and Seluanov, A. (2002). CLK-1 protein has DNA binding activity specific to O(L) region of mitochondrial DNA. FEBS Lett 516, 279-84. [Abstract]
Relevant LinksWormBase: http://www.wormbase.org/perl/ace/elegans/gene/locus?name=clk-1
LocusLink: http://www.ncbi.nih.gov/LocusLink/LocRpt.cgi?l=175729
Keywordsmetabolism, signaling, mitochondria, Caenorhabditis, elegans, worm, coenzyme Q, development, rhytmic behaviors