InterventionNicotinamide
Alternate NameNiacinamide
OrganismHomo sapiens (cell culture)
Aging PhenotypeNo effect on life-span
Allele TypeN/A
StrainIMR-90 and BJ fibroblasts
DescriptionAddition of nicotinamide to the culture media at 2-10 mM has no effect on replicative capacity of human fibroblasts, but does result in delayed onset of senescence associated phenotypes such as changes in cell morphology and expression of senscence-associated beta-galactosidase activity (Matuoka et al., 2001)
Gene FunctionNicotinamide (niacinamide) is a precursor of NAD.
Other PhenotypesHistone acetyltransferase activity is elevated in fibroblasts treated with nicotinamide (Matuoka et al., 2001). Nicotinamide inhibits NAD-dependent deacetylation of p53 by mSir2alpha (Luo et al., 2001).
HomologsN/A
Primary ReferenceMatuoka, K., Chen, K. Y., and Takenawa, T. (2001). Rapid reversion of aging phenotypes by nicotinamide through possible modulation of histone acetylation. Cell Mol Life Sci 58, 2108-16. [Abstract]
Other ReferencesLuo, J., Nikolaev, A. Y., Imai, S., Chen, D., Su, F., Shiloh, A., Guarente, L., and Gu, W. (2001). Negative control of p53 by Sir2alpha promotes cell survival under stress. Cell 107, 137-48. [Abstract]
Relevant Links
Keywordshuman, cell culture, fibroblast, NAD, histone deacetylase, SIR2, nicotinamide