|Organism||Homo sapiens (cell culture)|
|Aging Phenotype||Life-span extension|
|Strain||IMR190 lung fibroblast|
|Description||Addition of N-hydroxylamines (NHAs) such as N-t-butyl hydroxylamine (NtBHA), N-methyl hydroxylamine (NMHA), N-benzyl hydroxylamine (NBHA), or alpha-phenyl-N-t-butyl nitrone (PBN) resulted in an increase in replicative life-span of cultured fibroblasts (Atamna et al., 2000).|
|Other Phenotypes||Addition of NHAs reduced oxidant production and increased the ratio of reduced to oxidized glutathione in cells (Atamna et al., 2000). The corresponding O-hydroxylamines have no effects on life-span or oxidant production.|
PBN has been reported to extend life-span in mice but not in houseflies.
|Primary Reference||Atamna, H., Paler-Martinez, A., and Ames, B. N. (2000). N-t-butyl hydroxylamine, a hydrolysis product of alpha-phenyl-N-t-butyl nitrone, is more potent in delaying senescence in human lung fibroblasts. J Biol Chem 275, 6741-8. [Abstract]|
|Keywords||oxidative stress, human, cell culture, fibroblast, reactive oxygen species, oxygen free radicals, spin-trap|