InterventionN-t-butyl hydroxylamine
Alternate Name
OrganismHomo sapiens (cell culture)
Aging PhenotypeLife-span extension
Allele TypeN/A
StrainIMR190 lung fibroblast
DescriptionAddition of N-hydroxylamines (NHAs) such as N-t-butyl hydroxylamine (NtBHA), N-methyl hydroxylamine (NMHA), N-benzyl hydroxylamine (NBHA), or alpha-phenyl-N-t-butyl nitrone (PBN) resulted in an increase in replicative life-span of cultured fibroblasts (Atamna et al., 2000).
Gene FunctionN/A
Other PhenotypesAddition of NHAs reduced oxidant production and increased the ratio of reduced to oxidized glutathione in cells (Atamna et al., 2000). The corresponding O-hydroxylamines have no effects on life-span or oxidant production.
Primary ReferenceAtamna, H., Paler-Martinez, A., and Ames, B. N. (2000). N-t-butyl hydroxylamine, a hydrolysis product of alpha-phenyl-N-t-butyl nitrone, is more potent in delaying senescence in human lung fibroblasts. J Biol Chem 275, 6741-8. [Abstract]
Other References
Relevant Links
Keywordsoxidative stress, human, cell culture, fibroblast