InterventionRepeated Mild Heat Shock
Alternate Name
OrganismHomo sapiens (cell culture)
Aging PhenotypeNo effect on life-span
Allele TypeN/A
Strainhuman skin fibroblast
DescriptionExposure of fibroblasts to repeated mild heat shock (RMHS: 41C for 1hour twice per week) reduces the accumulation of advanced glycolation end products (AGE) and oxidized proteins with replicative age (Verbeke et al., 2001). RMHS has no effect on replicative life-span and fails to delay the onset of senescence associated phenotypes such as changes in cell morphology and size, lengthened cell doubling time, and expression of senescence associated beta-galactosidase.
Gene FunctionN/A
Other PhenotypesRMHS resulted in increased levels of reduced glutathione and decreased ratio of oxidized:reduced glutathione in both early and late passage cells (Verbeke et al., 2001).
Primary ReferenceVerbeke, P., Clark, B. F., and Rattan, S. I. (2001). Reduced levels of oxidized and glycoxidized proteins in human fibroblasts exposed to repeated mild heat shock during serial passaging in vitro. Free Radic Biol Med 31, 1593-602. [Abstract]
Other References
Relevant Links
Keywordsheat shock, temperature, replicative senescence, cell culture, human fibroblast