OrganismHomo sapiens (cell culture)
Aging PhenotypeLife-span extension
Allele TypeOverexpression
Strainhuman foreskin fibroblasts, retinal pigment epithelial cells
DescriptionTelomerase-expressing clones maintained normal length telomeres and continued to divide vigorously, in contrast to their telomerase-negative counterparts (Bodnar et al., 1998).
Gene FunctionCatalytic subunit of telomerase
Other PhenotypesClones of cells expressing telomerase also showed reduced staining for beta-galactosidase, a biomarker associated with cellular senescence (Bodnar et al., 1998). Expression of hTERT is also able to prevent the accelerated replicative senescence observed in cells taken from Werner’s patients (Wyllie et al., 2000).
HomologsS.c. EST2
S.p. Trt1
Primary ReferenceBodnar, A. G., Ouellette, M., Frolkis, M., Holt, S. E., Chiu, C. P., Morin, G. B., Harley, C. B., Shay, J. W., Lichtsteiner, S., and Wright, W. E. (1998). Extension of life-span by introduction of telomerase into normal human cells. Science 279, 349-52. [Abstract]
Other ReferencesVaziri, H., and Benchimol, S. (1998). Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr Biol 8, 279-82. [Abstract]
Wyllie, F. S., Jones, C. J., Skinner, J. W., Haughton, M. F., Wallis, C., Wynford-Thomas, D., Faragher, R. G., and Kipling, D. (2000). Telomerase prevents the accelerated cell ageing of Werner syndrome fibroblasts. Nat Genet 24, 16-7. [Abstract]
Relevant LinksLocusLink:
KeywordsHomo sapiens, human, telomeres, cancer, replicative senescence