OrganismSaccharomyces cerevisiae (replicative)
Aging PhenotypeLife-span extension
Allele TypeRecessive
DescriptionThe cdc6-1 conditional allele results in an approximately 20% increase in mean life span (Sinclair and Guarente, 1997). At 27C, this allele is defective for replication initiation from the rDNA ARS, resulting in a reduced rate of extrachromosomal rDNA circle accumulation (Sinclair and Guarente, 1997).
Gene FunctionRequired for pre-replicative complex formation (Cocker et al., 1996). Required for replication initiation (Bueno and Russell, 1992) and entry into S-phase (Zhou et al., 1989)
Other Phenotypescdc6-1 allele results in genomic instability at the permissive temperature (Bruschi et al., 1995).
HomologsS.c. ORC1
S.p. cdc18, orc1
C.e. Y39A1A.12, C43E11.10
D.m. CG5971, ORC1
M.m. CDC6, MM.42024
H.s. CDC6, ORC1L
Primary ReferenceSinclair, D. A., and Guarente, L. (1997). Extrachromosomal rDNA circles--a cause of aging in yeast. Cell 91, 1033-42. [Abstract]
Other ReferencesBruschi, C. V., McMillan, J. N., Coglievina, M., and Esposito, M. S. (1995). The genomic instability of yeast cdc6-1/cdc6-1 mutants involves chromosome structure and recombination. Mol Gen Genet 249, 8-18. [Abstract]
Bueno, A., and Russell, P. (1992). Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division. Embo J 11, 2167-76. [Abstract]
Cocker, J. H., Piatti, S., Santocanale, C., Nasmyth, K., and Diffley, J. F. (1996). An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature 379, 180-2. [Abstract]
Zhou, C., Huang, S. H., and Jong, A. Y. (1989). Molecular cloning of Saccharomyces cerevisiae CDC6 gene. Isolation, identification, and sequence analysis. J Biol Chem 264, 9022-9. [Abstract]
Relevant LinksYPD:
KeywordsrDNA, DNA replication, replicative senescence, Saccharomyces, cerevisiae, yeast, cell cycle