NameRAD52
OrganismSaccharomyces cerevisiae (replicative)
Aging PhenotypeShortened life-span
Allele TypeDeletion
StrainPSY316, W3031-A, W303R
DescriptionDeletion causes a 75% reduction in mean life span in strain PSY316 (Park et al., 1999). A similar reduction in life span is observed in strains W3031-A and W303R (M. Kaeberlein, M. McVey, and L. Guarente, unpublished).
Gene FunctionRequired for homologous recombination. Required for repair of nearly all double stranded DNA breaks (Malkova et al., 1996). Targets Rad51p to single-strand DNA (Song and Sung, 2000).
Other PhenotypesRequired for formation of extrachromosomal rDNA circles (Park et al., 1999), but not for replication fork pausing nor DNA breakage within the rDNA (Ivessa et al., 2000).
HomologsS.c. RAD59
S.p. rad29, rti1
M.m. RAD52
H.s. RAD52
Primary ReferencePark, P. U., Defossez, P. A., and Guarente, L. (1999). Effects of mutations in DNA repair genes on formation of ribosomal DNA circles and life span in Saccharomyces cerevisiae. Mol Cell Biol 19, 3848-56. [Abstract]
Other ReferencesIvessa, A. S., Zhou, J. Q., and Zakian, V. A. (2000). The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. Cell 100, 479-89. [Abstract]
Malkova, A., Ivanov, E. L., and Haber, J. E. (1996). Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication. Proc Natl Acad Sci U S A 93, 7131-6. [Abstract]
Song, B., and Sung, P. (2000). Functional interactions among yeast Rad51 recombinase, Rad52 mediator, and replication protein A in DNA strand exchange. J Biol Chem 275, 15895-904. [Abstract]
Relevant LinksSGD: http://genome-www4.stanford.edu/cgi-bin/SGD/locus.pl?locus=RAD52
YPD: http://www.proteome.com/databases/YPD/reports/RAD52.html
KeywordsHomologous recombination, DNA damage, ERC, rDNA, yeast, replicative senescence, Saccharomyces, cerevisiae