NamePHB1
OrganismSaccharomyces cerevisiae (replicative)
Aging PhenotypeShortened life-span
Allele TypeDeletion
StrainS150-2B
DescriptionDeletion results in approximately 20% shorter lifespan. Deletion of both PHB1 and PHB2 results in a life span that is approximately 40% shorter than wild type. Phenotypic changes characteristic of aging cells may suggest that PHB1/PHB2 mutants undergo premature aging, not simply reduction in viability. These changes include lengthening of cell cycle and characteristic morphological changes (Coates et al., 1997).
Gene FunctionPhb1p/Phb2p complex inhibits degradation of proteins in the mitochondrial membrane (Steglich et al., 1999).
Other PhenotypesNo reduction in stress resistance or bulk growth rate. A strong defect in mitochondrial potential was detected in the phb1 phb2 double mutant., and a slight defect in the phb1 single mutant (Coates et al., 1997). Deletion of PHB1 is synthetically lethal with mutation of outer mitochondrial membrane proteins, Mdm12p, Mdm10p, or Mmm1p (Berger and Yaffe, 1998).
HomologsS.c. PHB2
S.p. Spac1782.06c, Spac1322.16
C.e. Y37E3.0, T24H7.1
D.m. CG15081, CG10691, 1(2)37Cc
R.n. RN.29754
M.m. PHB, BCAP37, EPB7.2
H.s. PHB, EPB72
Primary ReferenceCoates, P. J., Jamieson, D. J., Smart, K., Prescott, A. R., and Hall, P. A. (1997). The prohibitin family of mitochondrial proteins regulate replicative lifespan. Curr Biol 7, 607-10. [Abstract]
Other ReferencesBerger, K. H., and Yaffe, M. P. (1998). Prohibitin family members interact genetically with mitochondrial inheritance components in Saccharomyces cerevisiae. Mol Cell Biol 18, 4043-52. [Abstract]
Steglich, G., Neupert, W., and Langer, T. (1999). Prohibitins regulate membrane protein degradation by the m-AAA protease in mitochondria. Mol Cell Biol 19, 3435-42. [Abstract]
Relevant LinksSGD: http://genome-www4.stanford.edu/cgi-bin/SGD/locus.pl?locus=PHB1
YPD: http://www.proteome.com/databases/YPD/reports/phb1.html
Keywordsyeast, replicative senescence, Saccharomyces, cerevisiae, mitochondria